Options trading entails significant risk and is not appropriate for all investors. Investors should consider their investment objectives and risks carefully before investing. There is always the potential of losing money when you invest in securities or other financial products. Keep in mind that while diversification may help spread risk, it does not assure a profit or protect against loss in a down market. The past performance of a security, or financial product does not guarantee future results or returns. The value of securities may fluctuate and as a result, clients may lose more than their original investment. That means your assets are protected up to $500,000 in value, including $250,000 in any cash awaiting reinvestment.Īll investments involve risk, and not all risks are suitable for every investor. Trades in your Webull Advisors account are executed by Webull Financial LLC, a member of the Securities Investor Protection Corporation (SIPC). See additional information on the Disclosures webpage. Registration does not imply a level of skill or training. Webull Advisors is an Investment Advisor registered with and regulated by the SEC under the Investment Advisors Act of 1940. Crypto trading only available to US residents.Īdvisory accounts and services are provided by Webull Advisors LLC (also known as "Webull Advisors"). Please visit to see a list of crypto available to trade. Not all coins provided by Apex Crypto LLC are available to New York residents. Please ensure that you fully understand the risks involved before trading. Apex Crypto is not a registered broker-dealer or FINRA member and your cryptocurrency holdings are not FDIC or SIPC insured. Cryptocurrency trading is offered through an account with Apex Crypto. Similar to SIPC protection, this additional insurance does not protect against a loss in the market value of securities.Ĭryptocurrency execution and custody services are provided by Apex Crypto LLC (NMLS ID 1828849) through a software licensing agreement between Apex Crypto LLC and Webull Pay LLC. The coverage limits provide protection for securities and cash up to an aggregate of $150 million, subject to maximum limits of $37.5 million for any one customer’s securities and $900,000 for any one customer’s cash. Our clearing firm Apex Clearing Corp has purchased an additional insurance policy. An explanatory brochure is available upon request or at Our clearing firm, Apex Clearing Corp., has purchased an additional insurance policy. Webull Financial LLC is a member of SIPC, which protects securities customers of its members up to $500,000 (including $250,000 for claims for cash). Webull Financial LLC is a member of the Financial Industry Regulatory Authority ( FINRA), Securities Investor Protection Corporation ( SIPC), The New York Stock Exchange ( NYSE), NASDAQ and Cboe EDGX Exchange, Inc ( CBOE EDGX). Registration does not imply a certain level of skill or training.Securities trading is offered to self-directed customers by Webull Financial LLC, a broker dealer registered with the Securities and Exchange Commission (SEC). Aptus Capital Advisors is a Registered Investment Advisor (RIA) registered with the Securities and Exchange Commission and is headquartered in Fairhope, Alabama. Be sure to consult with a tax professional before implementing any investment strategy. Nothing on this website should be interpreted to state or imply that past results are an indication of future All investments involve risk and unless otherwise stated, are not guaranteed. This website is for informational purposes only and does not constitute a complete description of our investment services or performance. Aptus Capital Advisors website and its associated links offer news, commentary,and generalized research, not personalized investment advice. Information presented is for educational purposes only and does not intend to make an offer or solicitation for the sale or purchase of any securities. For more information about our firm, or to receive a copy of our disclosure form ADV and Privacy Policy contact us.
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The best man was so eloquent in his toast to the newlyweds, the maid of honor was hesitant to follow him. See eloquent meaning in Hindi, eloquent definition, translation and. His poise, charm, and eloquence made him a highly sought-after speaker. The word or phrase eloquent refers to expressing yourself readily, clearly, effectively. The writer was known for his eloquence and detailed research into his subject matter. She was an eloquent speaker who always kept the audience enraptured. Examples of eloquence in the following topics. Use articulate to describe someone who is able to communicate clearly and coherently. The quality of artistry and persuasiveness in speech or writing. Use eloquent to describe someone who communicates clearly, in a moving and emotional manner. (of actions, gestures, etc.) forcefully showing feeling or emotion. elegant eloquent As adjectives the difference between elegant and eloquent is that elegant is characterised by or exhibiting elegance while eloquent is fluently persuasive and articulate. When somebody is eloquent or delivers an eloquent speech, he or she is articulate and well-spoken. skilled in or showing fluent, forceful, and appropriate speech:an eloquent teacher. fine, moving languageĮxpression, rhetoric, articulation, diction, expressiveness, orationĪlthough eloquence is usually used to describe speech, it can actually refer to any form of discourse. the ability to use language well and convincingly 2. Maybe I was more eloquent than I thought.1. He gave an eloquent speech after dinner. ELOQUENT, adjective Having the power of oratory speaking with fluency, propriety, elegance and animation as an eloquent orator an eloquent preacher.She was an eloquent speaker, able to move and inspire audiences.The poem is full of eloquent phrases about the beauty of nature.Aunt Margaret presided over the table with placid contentment, urging them to eat with eloquent movements of the eyes and hands.Eloquent suggests clarity and power: an eloquent plea for disarmament. Archbishop Ralph's eloquent letter to the pope had had no effect. eloquent - WordReference English dictionary, questions, discussion and forums.(adj) eloquent expressing yourself readily. It shouldnt because it encourages bad use of the casts property which exists to describe what you want to cast it to.Saying I want to cast a JSON field to JSON is nonsense but lots of people are using it. Explanation of the print with representation of Eloquence in French. Few will forget his eloquent defence of individual freedom. You are correct that it should be array but sadly json does actually work.At the press conference next morning, there was eloquent body-language.Justice Brennan offered an extraordinarily eloquent and diplomatic response.She always spoke with passion and eloquence. You need to express yourself with clarity, eloquence, and force. See eloquent More examples He is a man of charm, intelligence, and rare eloquence. His wit and eloquence made it a joy to be around him. It's often said that the world wants to silence the eloquent. the quality of delivering a clear, strong message: She was renowned for her eloquence and beauty.Being eloquent requires your words to be smooth, clear, powerful, and. eloquently adverb - eloquence noun Examples from the Corpus eloquent When something is beautifully, gorgeously, perfectly said (or written), its eloquent. From Longman Dictionary of Contemporary English eloquent el‧o‧quent / ˈeləkwənt / adjective 1 EXPRESS able to express your ideas and opinions well, especially in a way that influences people an eloquent appeal for support 2 EXPRESS showing a feeling or meaning without using words The photographs are an eloquent reminder of the horrors of war. ELOQUENT (adjective) articulate (expressing yourself easily or characterized by clear expressive language) elocute (declaim in an elocutionary manner). Tranquility is well equipped for adventure with a range of water toys including Jet Skis, Wave Runners, SeaBobs and an electric surfboard. The tender garage houses two 10.5 metre Hodgson tenders – one sport and one limousine that can carry up to 12 guests and two crew each. Spanning five decks, Tranquility is packed with premium superyacht features, including a foredeck helipad, spa and wellness area and 20 metre swimming pool. Welcome to the Xplor Boatworks Store Here you will find all of our cool swag to wear on the water, around the town, and every other day of your life Use the code: FREE50 if you order 50. Tranquility has remained among the top 10 largest explorer yachts in the world since being it was first delivered in 2014. boAt Xplorer O2 boAt Xplorer O2 4. However, one thing has remained constant. The Explorers versatility is limitless: diving, fishing, spearfishing, waterskiing nice weather, light wind or strong sea No problem the Explorer is always. The yacht hit the headlines again in 2019 as the renamed Tranquility when it played host to Kylie Jenner, who celebrated her 22nd birthday on board. Damen Yachting confirms Palm Beach International Boat Show attendance. Since then, the yacht has enjoyed a colourful reputation, after being seized by Indonesian authorities at the request of the US Department of Justice in 2018. Discover our fleet of SeaXplorer expedition yachts and fleet of Yacht Support. The operation was coordinated by watch officers at the Irish Coast Guard’s marine rescue sub-centre on Valentia Island in Co Kerry.The 91.5m explorer yacht Tranquility was the first yacht built to PYC standard when it was first delivered in 2014 by Dutch yard Oceanco originally called Equanimity. This item: boAt Rockerz 510 Bluetooth Wireless Over Ear Headphones with Mic, upto 20 Hours Playback, 50MM Drivers, Padded Ear Cushions and Dual Modes (Furious Blue) 2,299.00 1,499. The boat was able to continue under its own power to harbour in Portumna accompanied by the RNLI lifeboat crew. An assessment of the vessel was also carried out in an effort to establish whether any damage had been caused. In the meantime, the RNLI lifeboat crew was able to tow the grounded vessel off the rocks and into deeper water. Coast Guard land teams then transported the six to Terryglass where they were expected to collect and brought to Portumna by road. Explorer BOAT RANGE Have a boat, you’re bound to travel The Quintrex range of Explorer Tinnies are perfectly suited for tender work, lake, river and estuary style fishing. Six people in all were safely taken on board the Coast Guard rescue boat and brought ashore. This vessel is totally unique in that it specialises in camping under the stars. From an evening cruise to an epic fishing odyssey, the Explorer lives up to. This boat takes you farther it’s a holiday in itself. They’re rugged workhorses, with a host of features to make cruising more comfortable. However, before this could be attempted, a number of passengers had to be transferred to the Coast Guard boat to take some weight off the stranded vessel. Kimberley Xplorer boat information is detailed beloiw. Our toughest, hardest working boats are back with more refinements and options than ever. It was decided that an effort would be made to tow the vessel off the rocks. In response to customer requests for something more robust than a RIB, it’s offering the Xplorer 4. READ MORE: Met Eireann predict miserable week ahead and no good news in long-term forecast Now, International Yacht Network has its own manufacturing division, Marine Dynamics. The lifeboat crew checked that everyone on board was safe and carried out an assessment of the scene before deciding on what action to take. New 2022 Xpress Xplorer XP180 Bass Side Console Fishing boat powered by a 70hp 996cc 4-cyl EFI F70LA Yamaha 4-Stroke outboard engine. On arrival at the scene, RNLI volunteers found that the vessel had run aground on a rock shoal. Explorer Elite 165 (sold) Sandwich, Kent, United Kingdom. Currency - AUD - Australian Dollar Sort Sort Order List View Gallery View Submit. After the alarm was raised, the Lough Derg RNLI lifeboat was tasked along with the Killaloe unit of the Irish Coast Guard. Explorer boats for sale 56 Boats Available. The incident occurred at around 2.00 pm at Gortmore south of Terryglass Co Tipperary at the northern end of the lake. The RNLI and Irish Coast Guard were involved in an operation this afternoon to assist the crew of a passenger vessel with 40 people on board after it ran aground on rocks on Lough Derg. With thanks to user null1024 for details on the app and website. While SunVox is freeware, the developer is seeking donations to help support his efforts, so if you like the program, please do consider donating. CE support is currently limited to only ARM based devices, with assumed platform support for Windows CE 3.0 and higher. SunVox by Alex Zolotov A small, fast and powerful modular synthesizer with pattern-based sequencer (tracker) Operating system: Windows SunVox is a free, convenient and extremely fast instrument for creating music, consisting of a powerful sequencer and a modular synthesizer. The development log also indicates that version 2.1 is currently in active development. Set porttil mini: Celular Sony Xperia XZ1: sony Tracker Alexander Zolotov SunVox. The Windows CE version of the app is free having been last updated to version 2.0e in March 2022. 1.5K Posts - See Instagram photos and videos from sunvox hashtag. For those looking to have a play, the user interface offers a WYSIWYG layout with an on-screen piano-keyboard style interface for adding notes. It supports multi-track, sampling, WAV export, effect and tone generation amongst a plethora of other advanced features that will appeal to composers - including support for connectivity to external synth units and keyboards. It is a tool for those people who want to compose music anywhere. From the developer: SunVox is a small, fast and powerful music sequencer with modular synthesizers. This free Mac application is a product of Alex Zolotov. The program is a modular, patten-based sequence synthesiser which allows the user to compose music on the go. SunVox for Mac lies within Audio & Video Tools, more precisely Music Production. SunVox Library License Note that the SunVox library itself has this license: You can use SunVox library freely, but the following text should be included in your products (e.g. Products by Alexander Zolotov Quick Product Select Name Price Updated SunVox Tracker by Alexander Zolotov 5. SunVox (Q4050365) Music tracker and modular synthesizer developed by Alexander Zolotov edit Statements instance of music tracker 0 references modular synthesizer 0 references image SunVox v19b. Nothing sounds like it and it's a 1938 synthesizer. I love the 'Virtual ANS' synth to convert light spectrum into audio. The program is a music synthesiser that runs on Windows, Mac, Linux, iOS, Android and of course, Windows CE! nightradio Latest News, Product Listings and Discussion for Alexander Zolotov. Alexander Zolotov is an old school programming genius. SunVox has is a fairly niche product that has existed since its original inception in 2008. Simply use the item from your inventory, and you’ll create a summoning sign that can be seen by other players. This lets other players summon you to their world-effectively starting a cooperative session. The first one you’ll want to snag is the Tarnished’s Furled Finger (found in the game’s tutorial), which allows you to create a summon sign for cooperative play. Our app is compatible with hundreds of the best PC games and available now.Before you can run around with other players in Elden Ring, you’ll need to pick up a few key items. What frame rate does Elden Ring run at? Avg FPSĪre you experiencing Elden Ring FPS drops and stutters? Want to know exactly how the game performs on your system? An FPS monitor is the first step in understanding how the parts in your gaming PC are actually performing in real-world conditions.ĭownload our free FPS Monitor via Overwolf to count your frame rates as you play, and test how tweaks to your settings can boost FPS and increase Elden Ring performance. We reference thousands of reports from PCGameBenchmark users running our FPS tracking app to tell you exactly how Elden Ring performs across a range of different settings and resolutions on the most popular PC gaming setups. Elden Ring FPS - what frame rate can you expect? We'll help you find the best deal for the right gear to run the game. Filter for Elden Ring graphics card comparison and CPU compare. Looking for an upgrade? Try our easy to use Elden Ring set up guides to find the best, cheapest cards. The best place to get the game is via Elden Ring Steam download.Įlden Ring will run on PC system with Windows 10 and upwards. You'll need 60GB of free storage to install the game, although you do not necessarily need to install it onto the best SSD for gaming, as installation onto solid state storage is not required by the developers. The Elden Ring game size is not too demanding for a 2022 title. It operates as a hotfix that builds on the previous patch, that specifically addresses the duration of 'Ash of War - Endure' and also fixes a bug for 'Incantation - Inescapable Fury'. The Elden Ring update 1.07.1was released on October 25, 2022. If you want to know how to beat the game then check this Elden Ring boss order list. There is no Elden Ring crossplay, so you won't be able to play online with your PlayStation or Xbox friends. The game uses Easy Anti-Cheat software to prevent hacking and malicious activity. Elden Ring multiplayer is similar to Dark Souls in that it features PvP gameplay and co-operative elements. Out the best Elden Ring settings guide on PCGamesN for all your needs. Settings menu to get a great balance of fidelity and performance. Gaming PCs, but there are some easy changes that can be made in its In its Elden Ring review PCGamesN describes it as an "achingly beautiful open world" that will "floor you time and time again." Elden Ring settings You'll undoubtedly need to exceed this if you want to use 4K resolution, HDR, or ray tracing – the latter of which will arrive in a post-launch patch. Meanwhile, an Nvidia GeForce GTX 1070 or AMD Radeon RX Vega 56 will satiate the game's GPU demands. If you want to boost fps, you'll need to aim higher, as the Elden Ring recommended system requirements instead require an Intel Core i7-8700K or AMD Ryzen 5 3600X processor and 16GB of RAM. You'll, of course, need to clear some space on your storage solution of choice, to the tune of 60GB. In terms of graphics cards, an AMD Radeon RX 580 or Nvidia GeForce GTX 1060 will be enough to get you going. You'll need a CPU that's at least as powerful as an AMD Ryzen 3 3300X or Intel Core i5-8400, paired with a surprisingly large 12GB of RAM. Can my PC run Elden Ring? Despite launching on PlayStation 4 and Xbox One consoles, the Elden Ring minimum system requirements are surprisingly high. FeaturesĪvira is a free antivirus and adware elimination tool. It is a valuable tool for removing PUPs ( Potentially Unwanted Programs) and hidden adware. This tool employs a sophisticated mechanism. It also includes quick scanning and installation procedures. It’s compatible with both Mac and Windows. Malwarebytes ADWCleaner is one of the most widely regarded adware removal solutions.
µTorrent (or uTorrent) is my favorite and one of the most popular BitTorrent clients available for Mac OS X. Learn More For a long time uTorrent was one of our favourite torrent clients for Mac, combining a lightweight UI with a solid feature set. Moreover, unlocking PRO features for BitTorrent comes with a paid annual account, which is a bit daunting compared to the other services. However, the fact that the interface is not extremely user-friendly, and the download statistics are usually limited come as a drawback when using this service. The possibility to map ports automatically when using an alternative to utorrent mac is a great advantage of BitTorrent, which is tied to the fact that the platform is in direct sync with the official BitTorrent website and can stream torrents in a matter of seconds. UTorrent How to Stop Seeding? List of the best uTorrent alternatives for Mac PROS and CONS. Is uTorrent Legal? Click “Install” to install the program. But the ad-packed, mobile app “upgrade” business model is a total turnoff in desktop software. You’ll also find support for DHT and PEX, and it’s plenty fast. The interface isn’t nearly as pleasant, but it’s easier to get greater technical insight into your downloads. Mac and OSX Downloads – A feature-packed torrent client if you don’t mind the ads However, it offers significantly more capabilities than Transmission. Usually commercial software or games are produced for sale or to serve a commercial purpose.Vers 1.8.7 utorrent download on El Capitan Even though, most trial software products are only time-limited some also have feature limitations. After that trial period (usually 15 to 90 days) the user can decide whether to buy the software or not. Trial software allows the user to evaluate the software for a limited amount of time. Demos are usually not time-limited (like Trial software) but the functionality is limited. In some cases, all the functionality is disabled until the license is purchased. Demoĭemo programs have a limited functionality for free, but charge for an advanced set of features or for the removal of advertisements from the program's interfaces. In some cases, ads may be show to the users. Basically, a product is offered Free to Play (Freemium) and the user can decide if he wants to pay the money (Premium) for additional features, services, virtual or physical goods that expand the functionality of the game. This license is commonly used for video games and it allows users to download and play the game for free. There are many different open source licenses but they all must comply with the Open Source Definition - in brief: the software can be freely used, modified and shared. Programs released under this license can be used at no cost for both personal and commercial purposes. Open Source software is software with source code that anyone can inspect, modify or enhance. Freeware products can be used free of charge for both personal and professional (commercial use). Freeware programs can be downloaded used free of charge and without any time limitations. Choose the desired drive letter from “ Assign the following drive letter” drop-down box and click on Next. On the Assign Drive Letter or Path screen, you can specify the drive letter. On Specify Volume Size screen, enter the desired size of the disk in text-box named, “ Simple volume size in MB”. The first screen shows details about the wizard. To do that, right-click on “ Disk 1” and click on “ New Simple Volume.” Once the disk is online, let us create a volume. To do that, right-click on “ Disk 1” and click on “ Initialize disk.” See the following image:Ī dialog box “ Initialize Disk” opens. Once the disk is online, we must initialize it so that the logical disk manager can access it. To do that, right-click on “ Disk 1” and click on Online. To use it first, we must bring it online. See the following image:Īs you can see in the above image, an unallocated partition has been created. To do that, open Control Panel -> Open Administrator Tools -> Computer Management ->Click on “ Disk management,” which is on the left pan of Computer Management Snap-in. Now to use the iSCSI virtual disk, we must initialize it. We have configured the iSCSI target server on “ SAN.DC.Local” hence, enter the DNS Name and click on “ Quick Connect.” See the following image:Īs you can see in the following image, the initiator has been connected to the iSCSI virtual disk. To connect to the iSCSI virtual disk, enter the DNS or IP Address of the iSCSI target server. Here you can specify the IP Address or DNS Name of the iSCSI target server OR iSCSI target portal. To configure the initiator, open the Server manager -> Click on tools and click on “ iSCSI initiator”. Open Control Panel -> Administrative Tools -> open Services -> Find “ Microsoft iSCSI Initiator Service” service. See the following image:Īlternatively, you can start the service from Administrative tools. See the following image:Ī message box opens that says, we must start the services on the computer, and the service will be started automatically.” Click on Yes. To start the service, open “ Server Manager” -> Click on “ Tools” and select “ iSCSI initiator”. Following is the setup that I have created on my workstation.īy default, the iSCSI service is stopped. Local” server and connect the virtual disk that is created on SAN.DC.Local. In the previous article, I have already configured the iSCSI target server and created a virtual disk on “ SAN.Dc.Local” server. It uses an IP-based Ethernet network to communicate with the iSCSI target server. Workstations and servers use it to mount the target-based volume as a local volume and can be managed accordingly. The iSCSI initiator (Internet small computer system interface) is used to connect to the iSCSI based external storage. Install iSCSI target role on windows server 2016Ĭreate a new iSCSI virtual disk and configure the access server In my previous article, Installing and configuring the iSCSI target Server on Windows Server 2016, I have explained the following: In this article, I am going to explain how we can configure the iSCSI initiator and use it to connect to the iSCSI virtual disk on the target server. In February 2018, Fitbit announced a partnership with Adidas to release an Adidas-branded Fitbit Ionic it was released on March 19, 2018. On February 13, 2018, Fitbit acquired Twine Health. On January 10, 2017, Fitbit acquired Romania-based smartwatch startup Vector Watch SRL. In October 2016, CEO James Park announced that the company was undergoing a major transformation from what he called a "consumer electronics company" to a "digital healthcare company." On December 6, 2016, Fitbit acquired assets from Pebble for $23 million. In May 2016, Fitbit acquired a wearable payment platform from smart credit card company Coin. In June 2015, the company became a public company via an initial public offering, raising $732 million. On March 5, 2015, Fitbit acquired fitness coaching app developer Fitstar for $17.8 million. In January 2015, the company successfully defended against a trademark lawsuit from Fitbug. In October 2007, it changed its name to Fitbit, Inc. in San Francisco, California on Maby James Park ( CEO) and Eric Friedman ( CTO). The company was founded as Healthy Metrics Research, Inc. The company has sold more than 120 million devices and has 29 million users in over 100 countries. In 2019, Fitbit was the fifth largest wearable technology company in shipments. The company was acquired by Google in January 2021. It produces wireless-enabled wearable technology, physical fitness monitors and activity trackers such as smartwatches, pedometers and monitors for heart rate, quality of sleep and stairs climbed as well as related software. Fitbit (stylized as fitbit) is an American consumer electronics and fitness company. This scaffold serves as a tunable cell microenvironment and supports the formation of microvascular networks. To study the role of macrophages in vessel development for regenerative medicine, a bioactive poly(ethylene glycol)-based hydrogel scaffold that is modified for integrin-mediated cell adhesion and biodegradation by matrix-metalloproteinases 2 and 9 is utilized. Macrophages may play a beneficial role in blood vessel development. This review will provide an overview of commonly used biomaterials for forming synthetic hydrogels for tissue engineering applications and techniques for modifying them to with bioactive properties to elicit the desired cell responses. Because many synthetic hydrogel materials are inherently bioinert, they minimize unintentional cell responses and thus are good candidates for long-term implantable grafts, patches, and organs. Among the various 3D biomimetic scaffolds, synthetic hydrogels have emerged as a highly tunable and tissue-like biomaterial well-suited for implantable tissue-engineered constructs. Many great strides have been made toward this goal using various three-dimensional (3D) tissue culture scaffolds and specific media conditions. It follows that in order to recapitulate physiological cell responses and develop constructs capable of replacing damaged tissue, we must engineer the cellular microenvironment very carefully. Each cue provided in the microenvironment dictates cellular behavior and, thus, overall potential to perform tissue and organ specific function. At its core, this microenvironment is composed of precise arrangements of cells that encourage homotypic and heterotypic cell-cell interactions, biochemical signaling through soluble factors like cytokines, hormones, and autocrine, endocrine, or paracrine secretions, and the local extracellular matrix (ECM) that provides physical support and mechanobiological stimuli, and further regulates biochemical signaling through cell-ECM interactions like adhesions and growth factor sequestering. These results show that complementary nondiffusing biochemical signals can be linked into PEG-DA hydrogels simultaneously using 'Catcher-based ligation strategies, thereby inducing more nuanced cell-material interactions.The specific microenvironment that cells reside in fundamentally impacts their broader function in tissues and organs. Endothelial cells seeded onto PEG gels presenting both RGDS-SC and QK-SnpC showed ∼50% of cells actively proliferating (defined as Ki67+), compared to ∼31% of cells seeded on gels presenting RGDS-SC alone. QK-SnpC added to cell culture media enhanced endothelial cell proliferation compared to a negative control, and was statistically indistinguishable from the positive control of 130 pM VEGF 165. QK-SnpC formed a spontaneous covalent bond with SnoopTag peptide with 40% reaction efficiency, both in solution, in a PEG gel containing SnoopTag peptide, and in a PEG gel with both SnoopTag and SpyTag sites. Sp圜atcher was appended with an N-terminal adhesion ligand RGDS to form RGDS-SC, and SnoopCatcher was appended with the vascular endothelial growth factor (VEGF)-mimetic peptide QK to form QK-SnpC. The twin, chemically orthogonal protein ligation domains, Sp圜atcher and SnoopCatcher, were used to link two engineered proteins into poly(ethylene glycol) (PEG) hydrogels in order to control both endothelial cell adhesion and material-mediated pro-mitotic stimulation. |
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